Effectiveness of Cryptosporidium spp. oocysts detection and enumeration methods in water and milk samples
نویسندگان
چکیده
منابع مشابه
Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...
متن کاملdetection and enumeration of cryptosporidium oocysts in environmental water samples by real-time pcr assay
introduction: the protozoan parasite, cryptosporidium spp., widely spreads in both raw and drinking waters. it is the causative agents of waterborne diarrhea and gastroenteritis in the world. in the present study, a molecular assay was used for the detection and quantification of cryptosporidium oocysts in environmental water samples. materials and methods: thirty surface water samples were col...
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Flow cytometric detection of Cryptosporidium oocysts in human stool samples.
Cryptosporidium parvum is an important pathogen that causes diarrhea in virtually all human populations. Improved diagnostic methods are needed to understand the risk factors, modes of transmission, and impact of cryptosporidiosis. In the present study, we fluorescently labeled and counted C. parvum oocysts by flow cytometry (FC) and developed a simple and efficient method of processing human s...
متن کاملRecovery and detection of Cryptosporidium parvum oocysts from water samples using continuous flow centrifugation.
Continuous flow centrifugation (CFC) was used in conjunction with immunomagnetic separation (IMS) and immunofluorescence microscopy (IFA) and nested PCR to recover and detect oocysts of Cryptosporidium parvum and cysts of Giardia intestinalis from 10L volumes of source water samples. Using a spiking dose of 100 oocysts, nine of 10 runs were positive by IFA, with a mean recovery of 4.4+/-2.27 oo...
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ژورنال
عنوان ژورنال: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
سال: 2006
ISSN: 0102-0935
DOI: 10.1590/s0102-09352006000300023